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small scale bioconversion  (Eppendorf AG)


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    Eppendorf AG small scale bioconversion
    <t>Bioconversion</t> of xylan to XOS by MBP- Bs XynA11 and Bs XynA11-His. TLC analysis of XOS converted by MBP- Bs XynA11 (A) and Bs XynA11-His (B) after 1–48 h conversion. XOS standards (X1–X6) and control (B = blank) are shown. The corresponding XOS bioconversion yield (%) and concentration (g/L) for MBP- B sXynA11 (C) and B sXynA11-His (D), based on HPLC quantification after 48 h. Columns represent yield, while concentration is shown as the lines. Profile of XOS released from beechwood xylan by MBP- B sXynA11 (E) and B sXynA11-His (F) under varying reaction conditions. Enzymatic hydrolysis was performed using beechwood xylan concentrations of 1, 2, and 4% (w/v) and enzyme doses of 500–1500 U/mL at 40 °C and 900 rpm in citrate–phosphate buffer (pH 6.5) for 48 h. The resulting XOSs were quantified by HPLC using xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), and xylohexaose (X6) as standards (0–1.67 g/L).
    Small Scale Bioconversion, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/small+scale+bioconversion/pmc13022873-145-0-10?v=Eppendorf+AG
    Average 93 stars, based on 13 article reviews
    small scale bioconversion - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Production of Xylooligosaccharides (XOSs) with Anti-inflammatory and Prebiotic Activities Using Recombinant Endo-1,4-β-xylanases from Bacillus subtilis"

    Article Title: Production of Xylooligosaccharides (XOSs) with Anti-inflammatory and Prebiotic Activities Using Recombinant Endo-1,4-β-xylanases from Bacillus subtilis

    Journal: Journal of Agricultural and Food Chemistry

    doi: 10.1021/acs.jafc.5c13633

    Bioconversion of xylan to XOS by MBP- Bs XynA11 and Bs XynA11-His. TLC analysis of XOS converted by MBP- Bs XynA11 (A) and Bs XynA11-His (B) after 1–48 h conversion. XOS standards (X1–X6) and control (B = blank) are shown. The corresponding XOS bioconversion yield (%) and concentration (g/L) for MBP- B sXynA11 (C) and B sXynA11-His (D), based on HPLC quantification after 48 h. Columns represent yield, while concentration is shown as the lines. Profile of XOS released from beechwood xylan by MBP- B sXynA11 (E) and B sXynA11-His (F) under varying reaction conditions. Enzymatic hydrolysis was performed using beechwood xylan concentrations of 1, 2, and 4% (w/v) and enzyme doses of 500–1500 U/mL at 40 °C and 900 rpm in citrate–phosphate buffer (pH 6.5) for 48 h. The resulting XOSs were quantified by HPLC using xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), and xylohexaose (X6) as standards (0–1.67 g/L).
    Figure Legend Snippet: Bioconversion of xylan to XOS by MBP- Bs XynA11 and Bs XynA11-His. TLC analysis of XOS converted by MBP- Bs XynA11 (A) and Bs XynA11-His (B) after 1–48 h conversion. XOS standards (X1–X6) and control (B = blank) are shown. The corresponding XOS bioconversion yield (%) and concentration (g/L) for MBP- B sXynA11 (C) and B sXynA11-His (D), based on HPLC quantification after 48 h. Columns represent yield, while concentration is shown as the lines. Profile of XOS released from beechwood xylan by MBP- B sXynA11 (E) and B sXynA11-His (F) under varying reaction conditions. Enzymatic hydrolysis was performed using beechwood xylan concentrations of 1, 2, and 4% (w/v) and enzyme doses of 500–1500 U/mL at 40 °C and 900 rpm in citrate–phosphate buffer (pH 6.5) for 48 h. The resulting XOSs were quantified by HPLC using xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), and xylohexaose (X6) as standards (0–1.67 g/L).

    Techniques Used: Control, Concentration Assay

    Lab-scale bioconversion of XOS from beechwood xylan by MBP- B sXynA11. Physical appearance and representative chemical structures of beechwood xylan (A) and lyophilized XOS products (B) following enzymatic hydrolysis. (C) Time-course TLC analysis of XOS production at 0.5–48 h using MBP- Bs XynA11 (500 U/mL) at 40 °C, pH 6.5. Standards (X1–X6) and blank (B) included; (D) HPLC chromatogram of XOS products showing retention times of individual components: xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), xylohexaose (X6), and unquantified oligomers with DP > 6. Reactions were conducted in 100 mL volumes with 2% xylan at 250 rpm for 48 h.
    Figure Legend Snippet: Lab-scale bioconversion of XOS from beechwood xylan by MBP- B sXynA11. Physical appearance and representative chemical structures of beechwood xylan (A) and lyophilized XOS products (B) following enzymatic hydrolysis. (C) Time-course TLC analysis of XOS production at 0.5–48 h using MBP- Bs XynA11 (500 U/mL) at 40 °C, pH 6.5. Standards (X1–X6) and blank (B) included; (D) HPLC chromatogram of XOS products showing retention times of individual components: xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), xylohexaose (X6), and unquantified oligomers with DP > 6. Reactions were conducted in 100 mL volumes with 2% xylan at 250 rpm for 48 h.

    Techniques Used:



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    small scale bioconversion  (Eppendorf AG)
    93
    Eppendorf AG small scale bioconversion
    <t>Bioconversion</t> of xylan to XOS by MBP- Bs XynA11 and Bs XynA11-His. TLC analysis of XOS converted by MBP- Bs XynA11 (A) and Bs XynA11-His (B) after 1–48 h conversion. XOS standards (X1–X6) and control (B = blank) are shown. The corresponding XOS bioconversion yield (%) and concentration (g/L) for MBP- B sXynA11 (C) and B sXynA11-His (D), based on HPLC quantification after 48 h. Columns represent yield, while concentration is shown as the lines. Profile of XOS released from beechwood xylan by MBP- B sXynA11 (E) and B sXynA11-His (F) under varying reaction conditions. Enzymatic hydrolysis was performed using beechwood xylan concentrations of 1, 2, and 4% (w/v) and enzyme doses of 500–1500 U/mL at 40 °C and 900 rpm in citrate–phosphate buffer (pH 6.5) for 48 h. The resulting XOSs were quantified by HPLC using xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), and xylohexaose (X6) as standards (0–1.67 g/L).
    Small Scale Bioconversion, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/small+scale+bioconversion/pmc13022873-145-0-10?v=Eppendorf+AG
    Average 93 stars, based on 1 article reviews
    small scale bioconversion - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Bioconversion of xylan to XOS by MBP- Bs XynA11 and Bs XynA11-His. TLC analysis of XOS converted by MBP- Bs XynA11 (A) and Bs XynA11-His (B) after 1–48 h conversion. XOS standards (X1–X6) and control (B = blank) are shown. The corresponding XOS bioconversion yield (%) and concentration (g/L) for MBP- B sXynA11 (C) and B sXynA11-His (D), based on HPLC quantification after 48 h. Columns represent yield, while concentration is shown as the lines. Profile of XOS released from beechwood xylan by MBP- B sXynA11 (E) and B sXynA11-His (F) under varying reaction conditions. Enzymatic hydrolysis was performed using beechwood xylan concentrations of 1, 2, and 4% (w/v) and enzyme doses of 500–1500 U/mL at 40 °C and 900 rpm in citrate–phosphate buffer (pH 6.5) for 48 h. The resulting XOSs were quantified by HPLC using xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), and xylohexaose (X6) as standards (0–1.67 g/L).

    Journal: Journal of Agricultural and Food Chemistry

    Article Title: Production of Xylooligosaccharides (XOSs) with Anti-inflammatory and Prebiotic Activities Using Recombinant Endo-1,4-β-xylanases from Bacillus subtilis

    doi: 10.1021/acs.jafc.5c13633

    Figure Lengend Snippet: Bioconversion of xylan to XOS by MBP- Bs XynA11 and Bs XynA11-His. TLC analysis of XOS converted by MBP- Bs XynA11 (A) and Bs XynA11-His (B) after 1–48 h conversion. XOS standards (X1–X6) and control (B = blank) are shown. The corresponding XOS bioconversion yield (%) and concentration (g/L) for MBP- B sXynA11 (C) and B sXynA11-His (D), based on HPLC quantification after 48 h. Columns represent yield, while concentration is shown as the lines. Profile of XOS released from beechwood xylan by MBP- B sXynA11 (E) and B sXynA11-His (F) under varying reaction conditions. Enzymatic hydrolysis was performed using beechwood xylan concentrations of 1, 2, and 4% (w/v) and enzyme doses of 500–1500 U/mL at 40 °C and 900 rpm in citrate–phosphate buffer (pH 6.5) for 48 h. The resulting XOSs were quantified by HPLC using xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), and xylohexaose (X6) as standards (0–1.67 g/L).

    Article Snippet: Small-scale bioconversion of XOS was conducted in a 1.5 mL Eppendorf tube.

    Techniques: Control, Concentration Assay

    Lab-scale bioconversion of XOS from beechwood xylan by MBP- B sXynA11. Physical appearance and representative chemical structures of beechwood xylan (A) and lyophilized XOS products (B) following enzymatic hydrolysis. (C) Time-course TLC analysis of XOS production at 0.5–48 h using MBP- Bs XynA11 (500 U/mL) at 40 °C, pH 6.5. Standards (X1–X6) and blank (B) included; (D) HPLC chromatogram of XOS products showing retention times of individual components: xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), xylohexaose (X6), and unquantified oligomers with DP > 6. Reactions were conducted in 100 mL volumes with 2% xylan at 250 rpm for 48 h.

    Journal: Journal of Agricultural and Food Chemistry

    Article Title: Production of Xylooligosaccharides (XOSs) with Anti-inflammatory and Prebiotic Activities Using Recombinant Endo-1,4-β-xylanases from Bacillus subtilis

    doi: 10.1021/acs.jafc.5c13633

    Figure Lengend Snippet: Lab-scale bioconversion of XOS from beechwood xylan by MBP- B sXynA11. Physical appearance and representative chemical structures of beechwood xylan (A) and lyophilized XOS products (B) following enzymatic hydrolysis. (C) Time-course TLC analysis of XOS production at 0.5–48 h using MBP- Bs XynA11 (500 U/mL) at 40 °C, pH 6.5. Standards (X1–X6) and blank (B) included; (D) HPLC chromatogram of XOS products showing retention times of individual components: xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), xylohexaose (X6), and unquantified oligomers with DP > 6. Reactions were conducted in 100 mL volumes with 2% xylan at 250 rpm for 48 h.

    Article Snippet: Small-scale bioconversion of XOS was conducted in a 1.5 mL Eppendorf tube.

    Techniques: